METHOD OF DECONTAMINATION OF SPECIMENS OF AQUEOUS ICE FOR BIOLOGICAL INVESTIGATIONS Russian patent published in 2010 - IPC G01N33/18 G01N33/487 G01N1/10 

FIELD: testing equipment.

SUBSTANCE: method provides preliminary mechanical cleaning of external surface of ice specimens from biological and chemical contaminations by germless tool under minus 15°C. Then ice segments are treated by acyclic saturated hydrocarbon under minus 15°C by means of their submergence in vessel with acyclic saturated hydrocarbon. As acyclic saturated hydrocarbon pentane is used. After that ice segments are submerged in Freon cooled to minus 15°C for removal of acyclic saturated hydrocarbon. Ice specimens are ozonised in air-tight aluminium box using germicide UV lamp during not less than 30 min under minus 15°C. After that ice is treated by disinfectant solution free from chlorine in air atmosphere containing less than 100 particles of size less than 0.5 mcm in cubic foot. Ice specimens are washed with pure water first by means of submerging, then under water flow. After that ice is placed in sterile vessels. Pure water has resistance 18 megohm, it contains dissolved organic carbon 1 mcg per litre and is additionally cleaned by membrane with pore size of 5 kDa.

EFFECT: method allows to enhance integrity of decontamination performance.

2 cl