FIELD: chemistry; biochemistry.
SUBSTANCE: invention relates to medicine, specifically to sanitary and clinical microbiology. Chromogenic selective culture medium DagChrom agar for isolating and identifying colon bacterium and other coliform bacteria contains the following per g/l of distilled water: pancreatic digest of casein - 14.0-16.0, microbiological agar - 14.0-16.0, phosphate buffer - 0.2-0.3, chromogenic mixture - 2-nitrophenyl β-D-galactopyranoside - 0.3-0.4 and 5-bromo-4-chloro-3-indole β-D-glucuronid of cyclohexyl ammonium salt - 0.5-0.6, common kilka dry nutrient broth - 5.0-10.0, paraaminobenzoic acid - 0.01-0.015, bile salts - 1.5-2.0, brilliant green - 0.0002-0.0003.
EFFECT: method improves differentiating properties and simplifies the process of preparing domestic media for isolating and identifying Ecoli and other coliform bacteria.
1 tbl, 3 ex
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Authors
Dates
2010-04-20—Published
2008-10-14—Filed