METHOD OF IDENTIFICATION OF IMMUNE BINDERS FOR SURFACE ANTIGENS OF CELLS Russian patent published in 2016 - IPC G01N33/537 C07K16/28 C12N5/781 C12N15/13 

FIELD: bioengineering.

SUBSTANCE: method of identification of the immune body that specifically connects with the interesting antigen on the cell surface providing the animal immunization by expression vector coding the antigen of the cell surface; ensuring of contact of cells containing the immune bodies of their surface and released from the animal subjected to the immunization. The immune bodies are linked with the first sorted mark, with cells expressing the antigen linked with second sorted mark; identification of the specific linking using the cell sorter as per presence of the first and second sorted marks in the cell complex; as well as identification of regions determining complementarity with the antigen (CDR) in the identified immune body, and CDR implantation to the framework region of the acceptor-type immune body.

EFFECT: invention suggests screening of the immune bodies specific in relation to the membrane-bound antigens, this can be further used in development of the therapeutic and diagnostic agents.

4 cl, 8 dwg, 5 tbl, 5 ex