METHOD FOR DETECTING BACTERIAL ANTIGENS Russian patent published in 2012 - IPC C12Q1/00 G01N33/53 G01N33/533 

FIELD: medicine.

SUBSTANCE: method involves preparing a biochip containing immobilised antibodies to various antigens, incubating the biochip with analysed cell suspension for 1-2 hours at room temperature, washing off the biochip from unconjugated cells and detecting an antigen-antibody reaction with using an UV light source. A bacterial cell suspension is taken in the concentration of min. 1x10⁵ microbial cell/ml inactivated in any method not destroying the analysed antigen and resuspended in a buffer solution containing Tris HCl 50 mM, 0.02 % Tween 20 and NaCl 50 mM and propidium iodide in the concentration of 1.0-2.0 mcg/ml and used to coat the biochip surface in an amount sufficient to coat all its segments.

EFFECT: invention provides more simple and cheap method to detect the presence of bacterial antigens.

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