METHOD OF PRODUCING VACCINE AGAINST BLUETONGUE OF LARGE AND SMALL CATTLE (VERSIONS) AND VACCINE AGAINST BLUETONGUE OF LARGE AND SMALL CATTLE Russian patent published in 2010 - IPC A61K39/12 

FIELD: medicine.

SUBSTANCE: group of inventions relates to veterinary, in particular to veterinary virology and biotechnology. In accordance with the first version method of producing vaccine against bluetongue of large and small cattle includes preparation of bluetongue virus inoculum, infecting animal cell culture with inoculum, cultivation of cell-virus suspension, separation of virus specific antigen with its further inactivation and preparation of target product. As animal cell culture, "ВНК"-21/13 cells are used. Infecting animal cells is carried out with infecting dose 0.001-0.002 tcd5q/cell. Cultivation of cell-virus suspension is carried out at pH 7.2-7.4 and temperature 36.5-37.5°C for 48-72 hours until amount of viable cells is 7-10%. After that cell-virus suspension is centrifuged at 2.5-3.5 thousand rev/min. Antifoam agent in final concentration 0.02-0.025% is added to supernatant. After that chlorophorm in final concentration 0.7-0.8% is introduced into cell-virus suspension with constant mixing. Mixing is performed for 1.5-2.0 hours at pH 7.2-7.4 and temperature 36.5-37.5°C. Then reaction mixture is incubated for 1.5-2.0 hours at temperature 36.5-37.5°C and centrifuged at 8000-9000 rev/min, antigen is separated in form of supernatant. Antigen is concentrated until infectious activity is reached on "ВНК"-21/13 cell culture 6.5-7.5 lg tcd₅₀/_cm ³ and in accordance with the first version of vaccine production adjuvant in final concentration 40-65% is added to antigen, and in accordance with the second version of vaccine production saponin in final concentration 0.0001-0.0005% or aluminium hydroxide or mixture of aluminium hydroxide and saponin taken with weight ratio 1:0.000004-0.0000625, in final concentration 8-25% are added to antigen. During vaccine production necessity to perform labour-intensive operations connected with washing and preparation of a large number of cultivation vessels and repeated box work with each of them is eliminated completely, amount of full-fledged immunogen from the same volume of virus-containing cell suspension increases.

EFFECT: vaccine prepared in accordance with invention, possesses great immunogenic potency and, therefore, will protect animals against bluetongue more reliably.

5 cl, 15 ex