METHOD FOR PRODUCTION OF PROTEIN-HYDROCARBON BIOLOGICALLY ACTIVE FODDER ADDITIVE Russian patent published in 2010 - IPC A23J1/18 A23J1/12 C12N1/16 C12N1/20 

FIELD: agriculture.

SUBSTANCE: method provides for treatment of grain pellets suspension with content of solid phase of at least 10 wt %, multi-enzyme composition in concentration of not more than 0.1% for at least 2 hrs at pH 5.0, temperature of at least 50°C and mixing for not more than 100 rpm. Multi-enzyme composition comprises xylanase with activity of at least 4000 units/g, cellulase with activity of at least 1000 units/g, neutral protease with activity of at least 100 units/g and β-gluconase with activity of at least 950 units/g. To ferment-lysate mineral components of yeast medium "P" are added, inoculum of daily culture of yeast Yarrowia lypolytica Y-2375 is introduced in amount of at least 1.0% (vol.) with titre of cells up to 10⁸ cl/ml. Cultivation is carried out under conditions of aeration for not more than 8 hr at the temperature of at least 30°C. Produced heterogeneous mass that consists of non-hydrolysed pellets and yeast is soaked for at least 12 hr at the temperature of at least 40°C. Mineral components of "MRS" medium are added to produced autolysate. Three-day daily culture of Lactobacillus casei B-7657 is introduced in amount of at least 0.5% (vol.) and maintained under anaerobic conditions at the temperature of at least 37°C for at least 15 hr.

EFFECT: method makes it possible to produce the product with protein content 30%, cellulose - 12%, lactic acid bacteria of at least 10⁷ CFU/g.

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