FIELD: medicine.
SUBSTANCE: proposed method of purification of human recombinant interferon beta-1b (rIFN beta-1b) provides for optimisation of processes of purification of inclusion bodies, their dissolution and refolding of target protein Purification of human rIFN beta-1b is conducted with zwittehent 3-14 as a main detergent for purification and dissolving of inclusion body of Ecoli with the following refolding of rIFN beta-1b. After that prechromatographic processing of a sample is conducted and after that chromatographic processing of Ceramic S and of Source S30 and oxidation using a mixture of cysteamine/cystamine are performed. Then gel-filtration on Superdex 75 Prep grade, chromatographic processing on Source Q30, gel-filtration on Sephacril S200HR are made and finally the effectiveness of purification of the target protein by high performance liquid chromatography is monitored.
EFFECT: raise of the target protein yields and obtaining human rIFN beta-1b which meets the requirements of normative documents with high levels of specific antiviral activity.
5 tbl, 3 ex
| Title | Year | Author | Number |
|---|---|---|---|
| INDUSTRIAL METHOD OF PRODUCING AND PURIFYING RECOMBINANT HUMAN INTERFERON β-1b FROM INCLUSION BODIES | 2011 |
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RU2473696C1 |
| RECOMBINANT PLAZMIDNAJA DNA pTrcIFdL CODING POLYPEPTIDE WITH HUMAN GAMMA INTERFERON ACTIVITY AND Escherichia coli BACTERIA STRAIN - PRODUCER OF POLYPEPTIDE WITH HUMAN GAMMA INTERFERON ACTIVITY | 2009 |
|
RU2399670C1 |
| METHOD FOR PURIFYING RECOMBINANT PROTEIN OF TYPE III INTERFERON-LIKE FACTOR | 2012 |
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RU2549710C2 |
| METHOD OF PREPARING RECOMBINANT INTERFERON-ALPHA-2 FROM INSOLUBLE INCLUSION BODIES | 1996 |
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| PEG-MODIFIED HUMAN INTERFERON β-1a MOLECULE WITH ANTIVIRAL, IMMUNOMODULATORY AND ANTIPROLIFERATIVE ACTIVITY, WITH IMPROVED STABILITY, REDUCED IMMUNOGENICITY, IMPROVED PHARMACOKINETIC AND PHARMACODYNAMIC PARAMETERS, SUITABLE FOR MEDICAL USE, AND IMMUNOBIOLOGICAL FORMULATION BASED THEREON | 2013 |
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| EXPRESSION SYSTEM AND METHOD OF PRODUCING NON-MODIFIED RECOMBINANT PROTEINS IN Escherichia coli USING IT | 2015 |
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| STRAIN OF Escherichia coli BACTERIA - PRODUCENT OF HEAT SHOCK PROTEIN 70 AND METHOD FOR OBTAINING PREPARATION OF HUMAN HEAT SHOCK PROTEIN | 2013 |
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| RECOMBINANT PLASMID, Escherichia coli STRAIN, CHIMERIC PROTEIN AND THEIR APPLICATION | 2010 |
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| METHOD FOR PRODUCTION OF PROTEIN PREPARATION BELONGING TO STRESS GENUS AND HSP70 PROTEIN PREPARATION OBTAINED BY SAID METHOD | 2005 |
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| METHOD OF HUMAN RECOMBINANT GAMMA-INTERFERON PREPARING | 1997 |
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