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2 399 670

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IPC

C12N15/00(2006-01-01)

C12N15/23(2006-01-01)

C12N15/70(2006-01-01)

C12N1/21(2006-01-01)

C12R1/19(2006-01-01)

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Application

2009109152/13, 2009-03-13

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Start date

2009-03-13

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Actual filing date

2009-03-13

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Published

2010-09-20

(72)

Inventor

Shingarova Ljudmila NikolaevnaBoldyreva Elena FilippovnaTikhonov Roman VladimirovichJakimov Sergej AleksandrovichVul'Fson Andrej NikolaevichDolgikh Dmitrij AleksandrovichKirpichnikov Mikhail Petrovich

(73)

Holder

Uchrezhdenie Rossijskoj Akademii Nauk Institut Bioorganicheskoj Khimii Im. Akademikov M.M. Shemjakina I Ju.A. Ovchinnikova Ran

RECOMBINANT PLAZMIDNAJA DNA pTrcIFdL CODING POLYPEPTIDE WITH HUMAN GAMMA INTERFERON ACTIVITY AND Escherichia coli BACTERIA STRAIN - PRODUCER OF POLYPEPTIDE WITH HUMAN GAMMA INTERFERON ACTIVITY Russian patent published in 2010 - IPC C12N15/00 C12N15/23 C12N15/70 C12N1/21 C12R1/19

Abstract RU 2399670 C1

FIELD: medicine.

SUBSTANCE: recombinant plasmid DNA pTrcIFdL coding polypeptide with human gamma interferon activity of molar mass 3.06 Md (4.642 "т.п.о."), and physical map presented on dwg 1. is engineered. Said plasmid DNA pTrcIFdL is used to prepare BL21 (DE3)/pTrcIFdL strain, a producer of polypeptide with human gamma interferon activity.

EFFECT: invention allows preparing polypeptide with biological activity of human gamma interferon and improved thermal stability, and increasing its biosynthesis level.

2 cl, 2 dwg, 5 ex

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RU 2 399 670 C1

Authors

Shingarova Ljudmila Nikolaevna

Boldyreva Elena Filippovna

Tikhonov Roman Vladimirovich

Jakimov Sergej Aleksandrovich

Vul'Fson Andrej Nikolaevich

Dolgikh Dmitrij Aleksandrovich

Kirpichnikov Mikhail Petrovich

Dates

2010-09-20—Published

2009-03-13—Filed