METHOD OF ANTIOXIDANT ACTIVITY ASSESSMENT Russian patent published in 2010 - IPC G01N33/52 

FIELD: medicine.

SUBSTANCE: invention belongs to experimental and clinic medicine, notably to medical biophysics, clinic pharmacology, biochemistry, oncology and immunology. For antioxidant activity assessment, test specimen of an oxidant is injected into biological target, and then in target free-radical oxidation is induced. Antioxidant activity is assessed by optical density of the patterns within photometry. As biological target whole blood with addition of citrate or heparin is used. Test specimen of an oxidant is injected in 5% suspension made of whole blood, after incubation 10 mcl of 1 mM clotrimazole suspension is added. To induce oxidation 20 mcl of 100 mM tert-butyl hydroperoxide solution is added. Mix is once again incubated, centrifuged, and then supernatant's optical density is measured.

EFFECT: method allows increasing of accuracy and effectiveness of antioxidant activity assessment in natural and chemical compounds and biological liquids.

6 cl, 2 dwg