FIELD: agriculture.
SUBSTANCE: plasmid pC27RNS contains DNA of coding part of gene of extracell ribonuclease Zinnia elegans ZRNasell under control of inducible MAS2' -promotor. Plasmid pBiRNS contains DNA of coding part of gene of extracell ribonuclease Zinnia elegans ZRNasell under control of inducible 35S-promotor. Plasmid pC27RNS or pBiRNS is transferred into strain Agrobacterium tumefaciens. Produced agrobacteria are used to transform plant material by cocultivation with Agrobacteria and further selection of transgentic plants at selective medium.
EFFECT: expression of gene of extracell ribonuclease Zinnia elegans ZRNasell in plants gives them imprived resistance to virus infections.
3 cl, 3 dwg, 3 tbl, 5 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR PREPARING TRANSGENIC PLANTS RESISTANT TO VIRAL INFECTION | 2005 |
|
RU2300567C1 |
| PROCESS OF OBTAINING TRANSGENIC TOBACCO PLANTS WITH INCREAED CONTENT OF PROLINE | 2006 |
|
RU2324736C1 |
| CHIMERIC REGULATORY SITE FOR EXPRESSION OF GENES IN PLANTS (VARIANTS), CLASTER FOR GENE EXPRESSION (VARIANTS), CLASTER FOR INDUCIBLE EXPRESSION OF FOREIGN GENE (VARIANTS), METHOD OF GENE EXPRESSION IN PLANT (VARIANTS), METHOD OF INDUCIBLE EXPRESSION OF FOREIGN GENE IN PLANTS (VARIANTS) AND PLASMID (VARIANTS) | 1994 |
|
RU2142998C1 |
| PROCESS OF OBTAINING TRANSGENE TOBACCO PLANTS WITH INCREAED CONTENT OF PROLYL | 2006 |
|
RU2324737C1 |
| RECOMBINANT PLASMID DNA PG11 ENCODING SYNTHESIS OF HUMAN BETA-INTERFERON IN TRANSGENIC PLANTS AND A METHOD OF PRODUCING TRANSGENIC PLANTS SHOWING RESISTANCE TO VIRAL INFECTION | 1994 |
|
RU2103361C1 |
| RECOMBINANT pBM PLASMID AND METHOD OF OBTAINING NON-MARKER TRANSGENIC PLANTS, CAPABLE OF SYNTHESISING DESIRED PRODUCTS, USING SAID PLASMID | 2009 |
|
RU2410433C1 |
| METHOD OF GROWING TRANSGENIC CARROT PLANTS, WHICH PRODUCE HUMAN INTERLEUKIN-10 | 2007 |
|
RU2374321C2 |
| STRUCTURE OF DNA, STRUCTURE OF RECOMBINANT DNA AND METHOD OF PLANT PRODUCING | 1994 |
|
RU2136757C1 |
| RECOMBINANT PLASMID DNA pBi101-IL18 ENCODING HUMAN INTERLEUKIN-18 SYNTHESIS IN TRANSGENIC PLANTS | 2005 |
|
RU2302460C1 |
| TREHALOSE PRODUCING IN PLANTS | 1994 |
|
RU2143496C1 |
