PROCESS OF OBTAINING TRANSGENE TOBACCO PLANTS WITH INCREAED CONTENT OF PROLYL Russian patent published in 2008 - IPC C12N15/82 

FIELD: chemistry, organic, genetic engineering.

SUBSTANCE: invention relates to genetic engineering of plants. A recombinant plasmid DNA pBi-2E of the size 14099 bps will be constructed providing transfer of target DNA sequence into the plant genome and two-chain gene PDH suppressor expression consisting of the elements as follows: - DNA of the vector plasmid of the size 14758 bps; - DNA fragment containing the first exon of the PDH gene in direct orientation of the size 545 bps; - DNA fragment containing the first exon and the fragment of the first intron of the PDH gene of the size 691 bps in reverse orientation; - promoter 35S CaMV from the genome of the cauliflower mosaics virus' genome; - 3'HTP of the nopalinsynthase gene area. The plasmid pBi-2E will then be transferred to the strain Agrohacterittm tumefaciens AGL0 with subsequent direct transformation of the Nicotiana tabacum SR1 leaf discs with cultivation together with agribacteria. Selection and generation will be performed directly on the media containing NaCl. The process enables one to obtain transgenic tobacco plants using no antibiotics, possessing increased level of stress-resistance on account of their increased content of proline.

EFFECT: obtaining of transgenic tobacco plants using no antibiotics, possessing increased level of stress-resistance.

5 dgw, 1 tbl, 2 ex