FIELD: plant genetic engineering. SUBSTANCE: invention relates to preparing recombinant plasmid DNA pG11 encoding synthesis of β--interferon in plants showing resistance to viral infection. Plasmid pG11 has Eco RI-Bam HI-fragment of plasmid pSK-IFs including gene of human β--interferon. The obtained plasmid is inserted in agrobacterium strain and the latter is used for infection of plant leaf disks followed by testing the transformed cells for sensitivity to kanamycin. Full-value transgenic plants were regenerated on selective medium under condition of suspension culture. Plants show resistance to broad phytoviruses spectrum. EFFECT: improved method of transgenic plants producing. 2 cl, 4 dwg
| Title | Year | Author | Number |
|---|---|---|---|
| RECOMBINANT PLASMID DNA PG21 ENCODING HUMAN β-INTERFERON IN PLANTS | 1993 |
|
RU2035513C1 |
| METHOD FOR PREPARING TRANSGENIC PLANTS RESISTANT TO VIRAL INFECTION | 2005 |
|
RU2300567C1 |
| PROCESS OF OBTAINING TRANSGENE TOBACCO PLANTS WITH INCREAED CONTENT OF PROLYL | 2006 |
|
RU2324737C1 |
| PROCESS OF OBTAINING TRANSGENIC TOBACCO PLANTS WITH INCREAED CONTENT OF PROLINE | 2006 |
|
RU2324736C1 |
| RECOMBINANT PLASMID pBi121-ESAT6-CFP10-gIFN, CODING PROTEINS ESAT6 AND CFP10 MYCOBACTERIUM TUBERCULOSIS AND HUMAN GAMMA-INTERFERON IN TRANSGENIC PLANTS | 2011 |
|
RU2468082C1 |
| RECOMBINANT PLASMID DNA pBi101-IL18 ENCODING HUMAN INTERLEUKIN-18 SYNTHESIS IN TRANSGENIC PLANTS | 2005 |
|
RU2302460C1 |
| RECOMBINANT PLASMID, PROVIDING FOR EXPRESSION OF GENE OF EXTRACELL RIBONUCLEASE Zinnia elegans ZRNaseII IN TRANSGENIC PLANTS (VERSIONS) AND METHOD FOR PRODUCTION OF VIRUS-RESISTANT FORMS OF PLANTS | 2009 |
|
RU2393226C1 |
| METHOD OF GROWING TRANSGENIC CARROT PLANTS, WHICH PRODUCE HUMAN INTERLEUKIN-10 | 2007 |
|
RU2374321C2 |
| METHOD OF TRANSGENE PLANT PREPARING, RECOMBINANT PLASMID DNA PTI VY4 RESERVED FOR PREPARING OF TRANSGENE PLANTS, AND A STRAIN OF BACTERIUM AGROBACTERIUM TUMEFACIENS USED FOR PLANT TRANSFORMATION | 1988 |
|
SU1547316A3 |
| RECOMBINANT PLASMID PGBP450F FOR PREPARING TRANSGENIC PLANTS AND METHOD FOR PREPARING TRANSGENIC TOBACCO PLANTS WITH ENHANCED PRODUCTIVITY AND RESISTANCE TO FUNGAL PHYTOPATHOGENS | 2002 |
|
RU2237717C2 |
