FIELD: biotechnology, in particular plant gene engineering.
SUBSTANCE: recombinant plasmid DNA pBi101-IL18 is constructed having length of 15000 n.p. and containing DNA fragment of pBi101-IL18 vector plasmide having length of 13900 n.p.; -NdeI-BamHI/BgIII DNA fragment being cDNA site of human IL-18 gene; -Nol-NdeI fragment of pET15b plasmide; encoding N-terminal polypeptide from six hystidine amino acids and site of thrombin enzyme hydrolysis, -5'URT region from genome of tobacco etch virus; double 35S CaMV promoter from genome of cauliflower mosaic virus; -3'URT region from genome of cauliflower mosaic virus. Method of present invention makes it possible to transfer nucleotide sequence of human IL-18 in plant genomic DNA.
EFFECT: method for production of mature human IL-18 having biological activity.
2 dwg, 1 tbl, 3 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF GROWING TRANSGENIC CARROT PLANTS, WHICH PRODUCE HUMAN INTERLEUKIN-10 | 2007 |
|
RU2374321C2 |
| RECOMBINANT PLASMID DNA pBinPLUS-ARS-EPSPS ENABLING GENE EXPRESSION OF AGROBACTERIAL 5-ENOLPIRUVOYL-SHIKIMATE-3-PHOSPHAT-SYNTHETASE IN TRANSGENE CHLORELLA MICROALGAE | 2009 |
|
RU2407794C1 |
| PROCESS OF OBTAINING TRANSGENE TOBACCO PLANTS WITH INCREAED CONTENT OF PROLYL | 2006 |
|
RU2324737C1 |
| PROCESS OF OBTAINING TRANSGENIC TOBACCO PLANTS WITH INCREAED CONTENT OF PROLINE | 2006 |
|
RU2324736C1 |
| RECOMBINANT PLASMID pBi121-ESAT6-CFP10-gIFN, CODING PROTEINS ESAT6 AND CFP10 MYCOBACTERIUM TUBERCULOSIS AND HUMAN GAMMA-INTERFERON IN TRANSGENIC PLANTS | 2011 |
|
RU2468082C1 |
| RECOMBINANT PLASMID DNA PG11 ENCODING SYNTHESIS OF HUMAN BETA-INTERFERON IN TRANSGENIC PLANTS AND A METHOD OF PRODUCING TRANSGENIC PLANTS SHOWING RESISTANCE TO VIRAL INFECTION | 1994 |
|
RU2103361C1 |
| RECOMBINANT PLASMID DNA PG21 ENCODING HUMAN β-INTERFERON IN PLANTS | 1993 |
|
RU2035513C1 |
| RECOMBINANT PLASMID, PROVIDING FOR EXPRESSION OF GENE OF EXTRACELL RIBONUCLEASE Zinnia elegans ZRNaseII IN TRANSGENIC PLANTS (VERSIONS) AND METHOD FOR PRODUCTION OF VIRUS-RESISTANT FORMS OF PLANTS | 2009 |
|
RU2393226C1 |
| FRAGMENT HTLV-I/II OF GENE GAG, RECOMBINANT PLASMID DNA PATLG 579, STRAIN OF BACTERIUM ESCHERICHIA COLI HB 101/PATLG 579 - PRODUCER OF POLYPEPTIDE G 579 | 1998 |
|
RU2149876C1 |
| RECOMBINANT PLASMID pAd-SM, CODING HUMAN PROTEINS SOX2 AND C-MYC, BEING BASE FOR PRODUCTION OF VIRULENT ADENOVIRUSES DESIGNED FOR PRODUCTION OF INDUCED PLURIPOTENT HUMAN CELLS | 2012 |
|
RU2495127C2 |
