METHOD OF DETECTING ENANTIOMERS OF PRIMARY AMINE COMPOUNDS Russian patent published in 2010 - IPC G01N31/00 G01N30/00 

FIELD: chemistry.

SUBSTANCE: according to the invention, amine compounds of general formula (I) H₂N - CR¹R²R³, where R¹, R² and R³ can be bonded together, forming a (bi)cyclic structure together with the carbon atom to which they are bonded, or are not bonded together, and each represents H, substituted or unsubstituted (cyclo)alkyl or aryl or alkylaryl or arylalkyl radical which does not or does contain O, N, S, P halogen, B, Si heteroatom(s) or functional groups based on said atoms, in the presence of water and "ОФА" are brought into with enantiomerically pure thiol selected from N-acyl derivatives of (S)-cysteine or oligopeptides and their mimetic compounds which contain cysteine. The following general formula (II), z¹ - c(z² z³) - (z⁰)_m - (CH₂)_m - C(O) - (Cys), where {Cys} is S-cysteine, natural or synthetic oligopeptide or their mimetic compound which contains S-cysteine with a free sulfhydryl group, -Z⁰ - is an O, S atom or substituted amino group, -Z¹ is a H atom, substituted or unsubstituted cycloalkyl, alkyl, aryl, alkylaryl or arylalkyl radical which does not or does contain O, N, F heteroatom(s), -Z² is a H atom, substituted or unsubstituted alkyl or aryl radical, -Z³ is H, F atom, hydroxy-, alkoxy-, NHOH, substituted amino group, -m equals 0 or 1, -n equals 0, 1 or 2, - such that, compared to unsubstituted N-acyl derivatives of S-cysteine, e.g. N-acetyl-S-cysteine, the formula (II) compound contains one or more additional chiral centres and/or one or more additional O, N, F heteroatoms and is called a "functionalised" chiral SH-reagent. After that quantitative determination of the formed stereoisomeric isoindoles is carried out using one physical-chemical analysis technique, e.g. from chromatographic analysis techniques.

EFFECT: increased information content of determination.

6 cl, 5 ex, 9 tbl, 1 dwg