FIELD: biotechnologies.
SUBSTANCE: The invention relates to genetic engineering, particularly to engineering enzymology, and may be used for biocatalytic transformations and preparation of chemical compounds. For stereoselective acylating of primary amino alcohols and stereoselective hydrolysis of N-acyl derivatives of primary amino alcohols they use the mutated penicillin acylase from E. coli in which the amino-acid residue 71 of beta chain is replaced with leucine or arginine, or the amino-acid residue 145 of alpha chain is replaced with leucine.
EFFECT: The invention allows to improve the catalytic properties of penicillin acylase, in particular, its catalytic activity and stereoselectivity, as well as to enhance the ability of the enzyme to catalyze the acylation reaction of primary amino alcohols.
6 tbl, 5 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF CATALYTIC PROPERTIES IMPROVEMENT OF PENICILLINACYLAZE FROM ESCHERICHIA COLI AND USE OF MUTANT PENICILLINACYLAZE | 2013 |
|
RU2576002C2 |
| E.coli PENICILLIN ACYLASE MUTANT WITH IMPROVED PROPERTIES | 2012 |
|
RU2564578C2 |
| METHOD OF SYNTHESISING PEPTIDES, INCLUDING BETA-LACTAM ANTIBIOTICS WITH APPLICATION OF PENICILLIN ACYLASE VERSION | 2012 |
|
RU2537845C2 |
| CHEMOENZYMATIC SYNTHESIS OF 2,5-DIKETOMORFOLINE DERIVATIVES | 2019 |
|
RU2696099C1 |
| METHODS AND MATERIALS FOR PRODUCING SIMVASTATIN AND RELATED COMPOUNDS | 2007 |
|
RU2447152C2 |
| OBTAINING 1,4-DIAMINOBUTANE | 2010 |
|
RU2577967C2 |
| METHOD OF DETECTING ENANTIOMERS OF PRIMARY AMINE COMPOUNDS | 2006 |
|
RU2394236C2 |
| METHOD OF STEREOSELECTIVE SYNTHESIS OF HETEROBICYCLIC ALCOHOL ENANTIOMER, PURE ALCOHOL ENANTIOMER, METHOD OF SYNTHESIS OF PIPERAZINE DERIVATIVE | 1993 |
|
RU2124506C1 |
| METHOD FOR PREPARING LIPOLYTIC ENZYME VARIANT AND LIPOLYTIC ENZYME (VARIANTS) | 1999 |
|
RU2235775C2 |
| USE OF LIPOLYTIC ENZYME IN FOOD INDUSTRY | 2005 |
|
RU2406759C2 |
