FIELD: bioengineering.
SUBSTANCE: method includes the structure change of penicillinacylaze from Escherichia coli by the replacement of amino-acid residue 145 of an alpha-chain by leucine or of amino-acid residue 71 of a beta-chain by leucine or arginine.
EFFECT: method results in the improved catalytic activity of penicillinacylaze in the reactions of enantioselective acidation of the primary amino groups of chemical compounds and the enantioselective hydrolysis of the N-acyl derivatives of the primary aminecompounds.
6 tbl, 5 ex
| Title | Year | Author | Number |
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| METHOD TO IMPROVE CATALYTIC PROPERTIES OF PENICILLIN ACYLASE | 2009 |
|
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| E.coli PENICILLIN ACYLASE MUTANT WITH IMPROVED PROPERTIES | 2012 |
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| METHOD OF STEREOSELECTIVE SYNTHESIS OF HETEROBICYCLIC ALCOHOL ENANTIOMER, PURE ALCOHOL ENANTIOMER, METHOD OF SYNTHESIS OF PIPERAZINE DERIVATIVE | 1993 |
|
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| METHOD OF ENANTIOMERIC ENRICHMENT OF A MIXTURE CONSISTING OF TWO ENANTIOMERIC CHIRAL AMINES | 1990 |
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| METHOD FOR PRODUCTION OF CHIRAL ALPHA-HALOALKANOIC ACIDS | 2018 |
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| METHODS AND MATERIALS FOR PRODUCING SIMVASTATIN AND RELATED COMPOUNDS | 2007 |
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| METHOD OF PRODUCING (S)-7,8-DIFLUORO-2,3-DIHYDRO-3-METHYL-4H-[1,4]BENZOXAZINE | 2010 |
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