METHOD OF OBTAINING POST-TEST LEUCOCONCENTRATE OF PERIPHERAL BLOOD OF MAMMALS Russian patent published in 2010 - IPC A61K35/14 

Abstract RU 2394584 C1

FIELD: medicine.

SUBSTANCE: invention relates to medicine and veterinary, in particular to blood diagnostics. Claimed is method of obtaining post-test leucoconcentrate, which includes functional test: in Ependorf type tube on 1.5 ml 0.05 ml of fresh heparinised blood is introduced, into the same tube introduced is 0.05 ml of 0.2% nitroblue tetrazolium (NBT) solution on phosphate salt buffer or 0.05 ml of suspension of particles for phagocytocis, after finishing functional test the following activities are performed: 1 ml of 3.5% acetic acid solution is added, thoroughly mixed, centrifuged for 5 minutes at 1500 revolutions per minute, supernatant liquid is removed, sediment is re-suspended in remaining liquid, and with even distribution is applied on microscope slide, after which it is dried and stained by any suitable method.

EFFECT: invention ensures improvement of functional properties of post-test leucoconcentrate.

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RU 2 394 584 C1

Authors

Stekol'Nikov Anatolij Aleksandrovich

Bokarev Aleksandr Vladimirovich

Dashaev Isa Vakhaevich

Dates

2010-07-20Published

2009-01-11Filed