METHOD FOR DETECTING PHAGOCYTIC ACTIVITY OF NEUTROPHILS IN PERIPHERAL BLOOD OF ALIVE ORGANISMS Russian patent published in 2004 - IPC

FIELD: medicine, immunology, allergology, pulmonology, dermatology, infectious microbiology, veterinary science.

SUBSTANCE: the method has been suggested to exclude the possibility for obtaining falsely overestimated and falsely underestimated results of detection due to creating the conditions for keeping functional properties of neutrophils in vitro for every concrete organism. Moreover, one should perform blood sampling with anticoagulant, prepare the mixture to study phagocytosis that contains leukocytes and phagocytosis object in the course of which one should, at first, introduce phagocytosis object into blood with anticoagulant 30 min after its sampling, not later followed by filling in two capillaries with the obtained mixture. Mixture should be treated to study phagocytosis due to incubation at 37 C for every capillary, moreover, one of them should be incubated for 30 min, another one - for 2 h, and due to centrifuging every capillary for 10 min at 1500 rot./min. Right after centrifuging one should isolate leukocytes due to separating each capillary at the border of leukocytic residue and erythrocytic residue sedimentation and removing leukocytic residue out of corresponding part of every separated capillary. Smears should be prepared out of leukocytic residues obtained after centrifuging to determine the number of phagocytosis-entering neutrophils upon every smear and total number of microbes absorbed by neutrophils. Phagocytosis values should be calculated, they are: phagocytic index, phagocytic number, coefficient of phagocytic number and index of neutrophilic bactericidity. The data obtained for phagocytosis parameters should be compared to normal ones to conclude either upon availability or absence of neutrophilic phagocytic activity lesions in peripheral blood. The method increases the number of ways to evaluate phagocytic blood activity.

EFFECT: higher accuracy of detection.

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