METHOD FOR DETERMINATION OF FUNCTIONAL ACTIVITY OF PHAGOCYTES (FAF) Russian patent published in 2006 - IPC G01N33/53 G01N33/569 

FIELD: medicine, namely immunology, allergology, dermatology, microbiology; veterinary.

SUBSTANCE: claimed method includes providing of control mixture (CM); blood sampling with anticoagulant; providing of mixture for phagocytosis investigation (PhIM) by introducing in tested blood sample preliminary prepared 0.1 % solution of chromogenic reagent, namely tetrasolium nitroblue (NTB) dissolved in 0.9 % sodium chloride solution; PhIM incubation; preparation of smears from incubated followed by determination of amount of formasan-positive phagocytes in percents based on total phagocyte amount and FAF evaluation by comparison obtained values with normal ones. To produce CM part of preliminary prepared NTB solution with volume of at least 0.05 ml is blended with yeast Saccharomyces cerevisiae and obtained CM is held for at least 6 min at temperature of (20±2)°C. Then CM color is analyzed and rest part of NTB solution is used for PhIM preparation only in presence of pink color in CM. Method of present invention makes it possible to increase accuracy of FAF determination by 25-33 %.

EFFECT: method for FAF determination with improved accuracy.

7 cl, 8 tbl