FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to pharmacological production and can be used for making purpurogallin. The method provides enzymic oxidation of pyrogallol with hydrogen peroxide added and following chromatographic purification of an end product. Scaphechinus mirabilis urchins placed in a bioreactor with sea water are used as a source of enzyme and hydrogen peroxide. Aqueous pyrogallol is added to the bioreactor to sea water concentration 0.4-0.6 mg/ml. Chromatographic purification is enabled on polychrome-1 with following elution of the end product from a sorbent with 40-60 % aqueous ethanol, vacuum stripping and crystallisation from aqueous ethanol.
EFFECT: invention allows for higher yield of purpurogallin.
1 ex, 3 dwg
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