FIELD: medicine, veterinary science.
SUBSTANCE: invention refers to veterinary science. A culture medium for pre-implantation embryos contains DMEM medium, inactivated blood serum of 6-7-month nanny-goats with blood content of IGF1 and IGF2 making 54.73 and 231.50 nmol/l respectively, glucose, streptomycin and penicillin, in the following ratio, wt %: inactivated blood serum of 6-7-month nanny-goats - 15.0-20.0; glucose - 0.40-0.45; streptomycin - 0.004-0.005; penicillin - 0.003-0.0035; DMEM medium - the rest.
EFFECT: application of the presented culture medium for pre-implantation of embryos leads to prolonged storage stability of an unfrozen embryo, and also improves engraftment of previously implanted embryos when transplanted from donor goats to recipients.
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Authors
Dates
2010-08-10—Published
2008-12-31—Filed