FIELD: medicine.
SUBSTANCE: method of avian ES cell culture provides for: a) suspension of the ES cells originated from a blastoderm disk of an embryonated non-incubated bird's egg in a culture medium containing a primary culture medium added with growth factors and animal serum; b) inoculation with the ES cell suspension produced at the stage a) of a layer of nutrient cells and further ES cell culture. The only growth factors added to said culture medium are insulin-like growth factor 1 (IGF-1) and ciliary neurotrophic growth factor (CNTF).
EFFECT: invention allows optimising the medium formulation for avian ES cell culture which enables maintaining the typical signs of the ES cells and prevent them from differentiation.
17 cl, 9 dwg, 4 tbl, 4 ex
