FIELD: chemistry.
SUBSTANCE: water sample containing synthetic food dyes is mixed with ammonium sulphate until achieving its concentration in the solution of 43 wt %. The mixture is extracted with a mixture of acetone - 2-propanol solvents in ratio of 60:40 wt % in volume ratio of the mixture of solvents to the aqueous solution equal to 1:10. The extract is separated. The mixture of dyes separated through thin layer chromatography on Silufol plates. The mobile phase used is a mixture of acetone-isobutyl alcohol-potassium hydroxide solution with concentration of 0.1 mol/dm3 in volume ratio of 0.5:1:0.5-0.7. Chromatographic separation takes not more than 60 minutes. The dyes are identified from colour and/or shift factor which is calculated using the formula: Rf = X/Xf, where X the shift front of the identified dye; Xf is the shift front of the solvent.
EFFECT: proposed method enables identification of six synthetic dyes E102 Tartrazine, E110 Sunset Yellow, E122 Azorubine, E124 Ponceau 4R, E129 Allura red, E132 Indigo Carmine separately or in a mixture using less toxic solvents and reduction of the detection level.
1 cl, 1 tbl, 2 ex
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Authors
Dates
2010-08-27—Published
2008-12-15—Filed