FIELD: medicine.
SUBSTANCE: viral particle consists of non-alphavirus structural elements and contains an alphavirus vector replication-defective by deletion or substitution by at least one transgene. Besides said particle contains structural elements not coded by an alphavirus vector genome. A method for preparing said particle according to the invention implies the expression in trans of the genes coding the non-alphavirus structural elements, and the alphavirus vector, in a cell line and then recovery of the viral particles found in the cell culture supernatant.
EFFECT: invention allows preparing the viral particles with lower recombination risk.
24 cl, 5 dwg, 3 tbl, 2 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR PRODUCING REPLICATIVE INFLUENZA VIRUS PARTICLES, CELL COMPOSITION (VERSIONS), CELL CULTURE COMPOSITION AND APPLICATION THEREOF | 2005 |
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| METHOD OF OBTAINING RECOMBINANT VESICULAR STOMATITIS VIRUS | 2022 |
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| VIRAL VECTORS WITH CONDITION-DEPENDENT REPLICATION AND THEIR USING | 2001 |
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| ALPHAVIRUS REPLICON PARTICLE | 2018 |
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| METHOD FOR TEMPORARY TRANSFECTION FOR PRODUCING RETROVIRUS | 2016 |
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| STABLE CELL LINES FOR RETROVIRUSES PRODUCTION | 2016 |
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| BIOPRODUCTION OF LENTIVIRAL VECTORS | 2016 |
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