ALPHAVIRUS REPLICON PARTICLE Russian patent published in 2023 - IPC C12N15/86 C12N7/01 

FIELD: biotechnology; virology.

SUBSTANCE: alphavirus replicon particle (ARP) for gene delivery is proposed, which includes (1-i) a capsid and/or a shell, including VEEV structural proteins, and (1-ii) a VEEV replicon, including a polynucleotide encoding non-structural alphavirus proteins nsp1, nsp2, nsp3 and nsp4, and at least one gene of interest, wherein said alphavirus capsid proteins contain a mutation in the nuclear localization signal (NLS), or (2-i) a capsid and/or envelope comprising CHIKV structural proteins, and (1-ii) a VEEV replicon comprising a polynucleotide encoding the non-structural VEEV proteins nsp1, nsp2, nsp3 and nsp4, and at least one gene of interest, wherein preferably CHIKV is the CHIKV 37997 strain or the OPY-1 strain and VEEV is the VEEV TC-83 strain. A method for obtaining alphavirus replicon particles is proposed.

EFFECT: right gene delivery system has been developed to achieve successful gene therapy.

18 cl, 21 dwg, 1 tbl