FIELD: biotechnologies.
SUBSTANCE: method for modular design of DNA aptamers capable of specific and high-affinity binding of thrombin, which have stabilised basic substructure, provides for assembly of their structure by modelling using a combination of three structural modules containing a quadruplex nucleic acid module, a duplex nucleic acid module and a nucleic acid module that binds them and has a non-canonical structure by determining tertiary structure with a spectral circular dichroism method with confirmation of the fact of formation of more stable G-quadruplex that is different from quadruplex structure of initial structural quadruplex module.
EFFECT: application of a modular design method allows improving assembly efficiency of antithrombin DNA-aptamers having increased stability under physiological conditions.
4 cl, 7 dwg, 3 ex
| Title | Year | Author | Number |
|---|---|---|---|
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|
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