METHOD FOR PREPARING ACTIVATED MONONUCLEAR LEUKOCYTES Russian patent published in 2010 - IPC A61K35/14 A61K38/20 A61K31/724 

FIELD: medicine.

SUBSTANCE: invention concerns microbiology and biotechnology. There is offered a method for prepared activated mononuclear leukocytes involving the use of interleukin-2 in combination with cyclodextrin for mononuclear cell activation, a lymphocyte and macrophage concentrate recovered from donor or patient blood is activated in vitro with a complex containing a pharmacopeia preparation of recombinant interleukin-2 within the range of suboptimal doses 50-100 MU in 1 ml of the culture medium and β-cyclodextrin in concentration 1×10^-5-1×10^-3 M in the culture medium at the molar ratio of components either 1:1, or 1:3, or 1:10, the lymphocyte and macrophage concentrate of donor or patient blood is processed in vitro with a complex containing a pharmacopeia preparation of recombinant interleukin-2 within the range of suboptimal doses 50-100 MU in 1 ml of the culture medium and β-cyclodextrin in concentration 1×10^-5-1×10^-3 M in the culture medium the molar ratio of components either 1:1, or 1:3, or 1:10.

EFFECT: invention provides the preparation of IL-2/CD-MNL with high cytotoxic activity to tumour cell lines; 10-fold reduction of IL-2 doses with preserved clinical effectiveness of the activated cells allows reducing the intensity of systemic by-effects of the preparation, and also the use of immunotherapy in debilitated oncological patients, including in the patients with a hyperreactive response to the IL-2 introduction.

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