FIELD: medicine.
SUBSTANCE: tumor cells culture is contacted with natural killer cells and cytotoxic T-cells isolated from peripheral blood using 8 CD Micro Beads, human CD 56 and Micro Beads, human reagents. They are saturated with deuterium by incubation and culturing in the presence of interleukin-2 at a concentration of 10,000 IU/ml and heavy water concentration 7.7% at 37°C for 72 hours. Application of this method allows to increase the cytotoxic activity of lymphocytes responsible for tumor immunity, by deuterium saturation resulting in release of more stable oxidizing agents in an antitumor response.
EFFECT: increased cytotoxic activity of lymphocytes in vitro.
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