FIELD: chemistry; biochemistry.
SUBSTANCE: invention relates to biotechnology. Prepared pancreatic gland of slaughter animals is ground to particle size of 7-11 mm and the extracted in acidified distilled water at 7-15°C while periodically stirring. The suspension is separated on a settling and filtering centrifuge with automatic unloading of the precipitate using a filter element with pore size of 1-5 mm. Separation of fractions after salting out ballast substances and tripsin is carried out using a filter element with pore size of 16-20 mcm with subsequent drying of the desired product by spraying or sublimation. Desalination is carried out through diafiltration on ultrafiltration apparatus using hollow fibres.
EFFECT: invention increases output of the purified preparation, its activity and storage life.
2 cl, 2 tbl, 2 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF TRYPSIN PREPARING | 1988 |
|
SU1628526A1 |
| METHOD FOR PRODUCTION OF PROTEOLYTIC PREPARATION FOR MEDICAL USE | 2015 |
|
RU2610669C1 |
| METHOD FOR PREPARING IMMUNOMODULATING PREPARATION FROM REINDEER THYMUS | 2001 |
|
RU2205013C1 |
| METHOD OF PREPARING CHOLESTEROL-ESTERASE, TRIPSIN, DEOXYRIBONUCLEASE, AND RIBONUCLEASE FROM BOVINE PANCREAS | 2005 |
|
RU2311455C2 |
| APPLICATION OF A FLOW-THROUGH FILTERING CENTRIFUGE FOR THE EXTRACTION OF LACTOFERRIN FROM DAIRY RAW MATERIALS | 2021 |
|
RU2780347C1 |
| METHOD OF HYALURONIDASE PREPARING | 1992 |
|
RU2027759C1 |
| MANUFACTURE METHOD OF BLOOD SERUM STANDARD POSITIVE PANEL FOR QUALITY CONTROL OF IMMUNOENZYMOMETRIC TEST SYSTEMS DESIGNED FOR DETECTION OF VIRUS-SPECIFIC ANTIBODIES TO VIRAL RESPIRATORY INFECTIONS IN BIOLOGICAL LIQUIDS OF BOVINE ANIMALS | 2008 |
|
RU2392331C2 |
| METHOD OF PREPARING OF PROTEOLYTIC COMPLEX | 1992 |
|
RU2034028C1 |
| METHOD OF TRYPSIN IMMOBILISATION | 2010 |
|
RU2437936C1 |
| METHOD OF MANUFACTURING NECROECTOMIC DRESSING | 1998 |
|
RU2145874C1 |
