FIELD: chemistry.
SUBSTANCE: invention relates to chromatography of proteins. Disclosed method of preparing an adsorbent involves reaction of solid calcium phosphate with a solution of orthophosphoric acid, obtaining a neutral suspension of brushite, adding an alkali solution to the said suspension and holding the obtained suspension until formation a suspension of hydroxyapatite. Calcium phosphate reacts with 3M orthophosphoric acid taken in equimolar amount with hydromodulus equal to 1:1.2. The brushite is treated with the alkali solution with hydromodulus equal to 1:3.
EFFECT: invention simplifies the process of producing hydroxyapatite and increases sorption capacity of the desired product.
2 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR PREPARING ADSORBENT FOR PROTEIN CHROMATOGRAPHY | 2002 |
|
RU2209665C1 |
| METHOD OF MAKING ADSORBENT FOR PURIFYING PROTEINS | 2007 |
|
RU2349377C1 |
| METHOD OF PRODUCING BRUSHITE POWDER | 2009 |
|
RU2431599C2 |
| METHOD OF PREPARING MIXTURE FOR PRODUCING CERAMIC BIODEGRADABLE MATERIAL | 2010 |
|
RU2456253C2 |
| PRODUCING METHOD OF NANOCRYSTALLINE SILICON-REPLACED HYDROXYAPATITE | 2012 |
|
RU2500840C1 |
| METHOD OF PRODUCING NANO-SIZED CALCIUM-DEFICIENT CARBONATE-CONTAINING HYDROXYAPATITE | 2014 |
|
RU2588525C1 |
| METHOD TO PREPARE CHARGE TO PRODUCE BIOCERAMICS | 2009 |
|
RU2431627C2 |
| METHOD FOR MAKING BIODEGRADABLE CERAMIC COMPOSITE OF DOUBLE POTASSIUM CALCIUM PHOSPHATE | 2008 |
|
RU2395303C1 |
| METHOD FOR PRODUCTION OF AFFINITY ADSORBENT FOR CELLULOLITIC ENZYME FRACTIONATION | 2005 |
|
RU2293571C1 |
| METHOD FOR PRODUCING HYDROXYAPATITE | 1993 |
|
RU2077475C1 |
