FIELD: biotechnology, biochemistry, proteins. SUBSTANCE: invention relates to chromatography of proteins and can be used for purification and fractionation of enzymes. Method for preparing hydroxylapatite adsorbent involves the usage of calcium insoluble salts for preparing brushite, for example, calcium carbonate or ortho-phosphate that are treated with excess of sodium (potassium) dihydrogen phosphate or ortho-phosphoric acid at pH = 1.0-4.0 at temperature 100 C. Also, method involves preparing spherical hydroxylapatite particles that includes carrying out stages for preparing brushite and hydroxylapatite in round-bottomed flask rotating at the rate 10-50 rev/min. Method provides reducing number of the process steps and to enhance the yield of coarse-grained fractions of hydroxylapatite. EFFECT: improved preparing method. 5 cl
