DEVICE AND METHOD OF LIQUID SAMPLE INTRODUCTION INTO FLUID CARRIER FLOW AND THEIR APPLICATION FOR NUCLEIC ACID AMPLIFICATION Russian patent published in 2010 - IPC G01N35/08 C12Q1/68 B01L7/00 

FIELD: medicine.

SUBSTANCE: device for liquid sample introduction into the fluid carrier flow running through a continuous flow tube having an outlet and a common inlet wherein there are introduced both a carrier flow, and a liquid sample; the device comprises a tank for continuous fluid carrier supply into the inlet; the tank is designed to maintain the constant fluid carrier level above the inlet and fluid connected with the continuous flow tube in such a manner that when used, the fluid carrier flow and liquid sample are absorbed through the continuous flow tube when the tank is under atmospheric pressure and when the fluid carrier represents a hydrophobic liquid, while the liquid samples represents water test. The method involves the use of the device described above, creation of fluid connection of the continuous flow tube inlet and the tank, introduction of the fluid carrier in the tank and introduction of the liquid sample in the fluid carrier; while the fluid carrier flow and liquid sample are absorbed through the continuous flow tube when the tank is under atmospheric pressure. Also the method can involve immersion of a liquid sample distributor in the fluid carrier which is contained in the tank, liquid sample distribution in the inlet and running of the distributed dosed liquid sample by the liquid sample distributor so to supply the distributed liquid sample into the inlet and to absorb it through the continuous flow tube. The fluid carrier flow and liquid sample are absorbed through the continuous flow tube when the tank is under atmospheric pressure. Also the group of inventions includes a continuous flow system which contains the device specified above, and a method of nucleic acid amplification in PCR or LCR format, with the use of specified system.

EFFECT: method improvement.

21 dwg, 4 ex, 8 cl