RECOMBINANT PROTEIN LACspCBD POSSESSING BETA-GALCTOSIDASE ACTIVITY AND ABILITY FOR SPONTANEOUS BINDING WITH CELLULOSE-CONTAINING SORBENTS, RECOMBINANT PLASMID DNA ENCODING SYNTHESIS OF RECOMBINANT PROTEIN LACspCBD, STRAIN Escherichia coli M15 [pREP4, pLACspCBD] AS PRODUCER OF RECOMBINANT PROTEIN LACspCBD, METHOD FOR PREPARING IMMOBILIZED RECOMBINANT PROTEIN LACspCBD ON CELLULOSE AND METHOD FOR ENZYMATIC CLEAVAGE OF LACTOSE Russian patent published in 2006 - IPC C12N9/38 C12N15/56 C12N15/72 C12N1/21 C12N11/12 C12P19/00 C12R1/19 

FIELD: genetic engineering, biotechnology.

SUBSTANCE: invention proposes a fused protein of beta-galactosidase and cellulose-binding domain of endoglucanase CelD from Anaerocellum thermophilium. The recombinant plasmid DNA pLACspCBD is constructed providing its expression in E. coli cells. The strain E. coli as a producer of indicated fused protein is obtained. A method for preparing this fused protein in immobilized form is developed that involves treatment of cellulose with hydrolyzate of the indicated strain of E. coli. Using the invention provides simplifying processing of dairy foodstuffs. Invention can be used in food processing industry.

EFFECT: valuable properties of strain and protein, improved preparing method.

5 cl, 1 dwg, 5 ex