METHOD FOR IMMOBILISING BIOLOGICALLY ACTIVE SUBSTANCE (BAS) ON CARRIER (VERSIONS) AND BAS-CARRIER CONJUGATE PRODUCED BY SUCH METHODS Russian patent published in 2011 - IPC C12N11/08 C12N11/10 A61K38/00 A61K38/43 

FIELD: medicine.

SUBSTANCE: biologically active substance (BAS) is dissolved in a water solution of polymer concentrated more than 10 % to concentration of a saturated solution. The prepared reaction mixture is exposed to ionising radiation. The second version of the method consists in the fact that the BAS is dissolved in a water solution of polymer, then the mixture is frozen to temperature (-20°)-(-140°) C and exposed to ionising radiation. A BAS-carrier conjugate prepared by the offered methods is characterised by the fact that the BAS is specified in a group: proinsulin, tyrozyl-2-alanyl-glycyl-phenylalanyl-leucyl-arginine diacetate, alkalase, hyaluronidase, somatotropin, and the carrier is specified in a group: polyethylene oxide, polyvinyl pyrrolidone, dextrane, pluronic, acrylamide. A BAS molecule is covalent bounded with at least one polymer molecule.

EFFECT: invention enables immobilisation on the polymer carrier of those water-soluble BAS which are unstable, destroyed when immobilised by the other methods completely or almost completely, and increase in a portion of the preserved BAS when the immobilised BAV is destroyed incompletely.

23 cl, 34 dwg, 3 tbl, 20 ex