FIELD: chemistry; biochemistry.
SUBSTANCE: invention relates to biotechnology, specifically to a method of growing reticulum cells from fragments of an unaltered human autologous lymph node and can be used to treat chronic lymphatic oedema. A donor (patient) undergoes biopsy of the pathologically unaltered surface lymph node which is heterotopic towards the affected organ. The obtained biopsy material is cleaned from capsules and subcutaneous fat, divided into fragments, washed and treated with a mixture of lytic enzymes. The fragments are thoroughly washed from enzymes in a full growth medium. Further, cells are grown in MEM medium for 4-5 weeks.
EFFECT: invention enables to obtain large amount of pure culture of autologous reticulum cells of the lymph node.
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF COMPENSATION OF JOINT CARTILAGE DEFECTS | 2008 |
|
RU2383310C1 |
| CELL GRAFT FOR TREATING DESTRUCTIVE DYSTROPHIC DISEASES AND TRAUMATIC INJURES OF ARTICULAR CARTILAGE | 2008 |
|
RU2402339C2 |
| CELL GRAFT FOR TREATING DISEASES AND TRAUMATIC INJURES OF ARTICULAR CARTILAGE | 2008 |
|
RU2402340C2 |
| METHOD OF STIMULATION OF FIBROPLASTS GROWTH IN CULTURE | 2005 |
|
RU2287013C1 |
| METHOD FOR MAKING LARGE-BLOCK FREEZE-DRIED BONE IMPLANTS | 2008 |
|
RU2366173C1 |
| METHOD OF MANUFACTURING INTRA-TISSUE POROUS IMPLANTS | 2009 |
|
RU2415655C1 |
| METHOD FOR PRODUCING AND CULTURING FIBROBLAST-LIKE CELLS FROM MILK TOOTH PULP | 2020 |
|
RU2726554C1 |
| METHOD FOR PRODUCING AND CULTURING FIBROBLAST-LIKE CELLS FROM A NEWBORN'S UMBILICAL CORD | 2019 |
|
RU2710263C1 |
| METHOD FOR TREATING ACUTE AND CHRONIC LYMPHATIC EDEMA OF LIMBS | 2004 |
|
RU2280461C2 |
| METHOD OF SEPARATING FIBROBLASTS, METHOD OF CREATING THEREOF-BASED BIOTRANSPLANT (VERSIONS) AND METHOD OF REGENERATING HUMAN TISSUES (VERSIONS) | 2013 |
|
RU2567004C2 |
