FIELD: chemistry; biochemistry.
SUBSTANCE: invention relates to genetic engineering. Disclosed are expression DNA structures which include a T-shaped DNA structure with a fragment (19-23 bases)of the target gene sequence subject to transcription with formation of siRNA, which provide specific inhibition of said gene in a cell via RNA interference. Described is a method of obtaining the disclosed DNA structure, which does not involve PCR steps and is a simple three-step procedure which is performed in a single reaction vessel.
EFFECT: invention can be used for practical purposes for inhibiting production of target proteins in cells.
14 cl, 7 dwg, 1 ex
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