METHOD FOR PRODUCING R-BRUCELLAR ERYTHROCYTIC DIAGNOSTICUM FOR INDIRECT HEMAGGLUTINATION TEST (IHGT) Russian patent published in 2011 - IPC A61K39/10 G01N33/531 

FIELD: medicine, veterinary science.

SUBSTANCE: invention refers to veterinary science. The method involves producing R-antigen of B.abortus 16/4 brucella strain, analysing its activity by titration, producing sheep's formalinised and tanninised erythrocytes and sensitising said sheep's formalinised and tanninised erythrocytes to titrated R-antigen. For producing R-antigen, a three-day culture of B.abortus 16/4 brucella strain is washed off from a surface of a nutrient medium with sterile physiologic saline and centrifuged. Precipitated microbial cells are mechanically destructed by thorough grinding, while and sensitising said sheep's formalinised and tanninised erythrocytes to titrated R-antigen is ensured by keeping their mixture at temperature 45-50°C for one hour.

EFFECT: method allows to produce high-activity and specificity R-brucellar erythrocytic diagnosticum.

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