METHOD TO PRODUCE ERYTHROCYTIC ANTIGEN FOR REACTION OF INDIRECT HEMAGGLUTINATION IN CASE OF BRUCELLOSIS Russian patent published in 2013 - IPC G01N33/569 

FIELD: veterinary science.

SUBSTANCE: method to produce erythocytic antigen for reaction of indirect hemagglutination in case of brucellosis includes preparation of formalinised erythrocytes of sheep and their sensibilisation with sensitin produced by growth of bacterial brucella mass, its washout with hypertonic solution of sodium chloride, inactivation, extraction and separation of sensitin by centrifugation. At the same time formalinised erythrocytes of sheep are sensibilised with antigen made by exposure of the inactivated bacterial mass of the strain B.abortus 19 in the dose of 40-50 billion microbial cells per 1 ml of sodium dodecyl sulfate in 1% concentration at 70-72°C for 45 minutes, with further loading of erythrocytes with sensitin in terms of 0.2-0.4 ml of antigen per 1 ml of 5% suspension of erythrocytes.

EFFECT: method improvement.

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