FIELD: veterinary medicine.
SUBSTANCE: method of production of R-brucellar erythrocytic antigen for indirect hemagglutination test (IHA) includes production of formalinised red blood cells of sheep, their sensibilisation with sensitine obtained by growing bacterial mass of brucella, its washing-off with the hypertonic solution of sodium chloride, inactivation, extraction and separation of sensitine by centrifugation. At that the antigen is taken out from the strain B.abortus 16/4 by acting on the bacterial cells with 0.5% concentration of sodium dodecyl sulfate at the temperature of 68-70°C for 60 minutes.
EFFECT: invention enables to produce a diagnostic preparation of high performance for diagnostics of brucellosis in animals.
5 tbl, 1 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD TO PRODUCE ERYTHROCYTIC ANTIGEN FOR REACTION OF INDIRECT HEMAGGLUTINATION IN CASE OF BRUCELLOSIS | 2011 |
|
RU2484481C1 |
| METHOD FOR PRODUCING R-BRUCELLAR ERYTHROCYTIC DIAGNOSTICUM FOR INDIRECT HEMAGGLUTINATION TEST (IHGT) | 2008 |
|
RU2411041C2 |
| METHOD OF PRODUCING A BRUCELLOUS ERYTHROCYTE ANTIGEN FOR THE INDIRECT HEMAGGLUTINATION REACTION (IHAR) | 2019 |
|
RU2714305C1 |
| METHOD FOR OBTAINING ERYTHROCYTIC ANTIGEN FOR REACTION OF INDIRECT HEMAGGLUTINATION IN CASE OF BRUCELLOSIS | 2005 |
|
RU2283498C1 |
| METHOD FOR PRODUCING BRUCELLAR ERYTHROCYTIC DIAGNOSTICUM FOR INDIRECT HEMAGGLUTINATION TEST (IHGT) | 2016 |
|
RU2667121C2 |
| METHOD FOR PRODUCING ERYTHROCYTIC ANTIGEN FOR SERUM DIAGNOSTICS OF BRUCELLOSIS | 2008 |
|
RU2415434C2 |
| METHOD OF OBTAINING ERYTHROCYTE DIAGNOSTICUM FOR REACTION OF INDIRECT HEMAGGLUTINATION IN CATTLE COCCIELLOSIS | 2009 |
|
RU2410698C1 |
| METHOD FOR PREPARING ANTIBODY ERYTHROCYTE DIAGNOSTICUM FOR INDIRECT HEMAGGLUTINATION TEST (IHT) FOR PURPOSES OF BRUCELLA INDICATION IN PATHOLOGIC MATERIAL | 2012 |
|
RU2493871C1 |
| METHOD FOR PREPARING ERYTHROCYTIC DIAGNOSTICUM FOR INDIRECT HEMAGGLUTINATION TEST (IHGT) IN CATTLE ANAPLASMOSIS | 2007 |
|
RU2366453C2 |
| METHOD FOR OBTAINING ERYTHROCYTIC ANTIBODY OVIS DIAGNOSTICUM FOR INDIRECT HEMAGGLUTINATION REACTION (IHAR) TO INDICATE OVIS ANTIGEN IN BIOMATERIAL | 2012 |
|
RU2509306C2 |
