FIELD: medicine.
SUBSTANCE: method of obtaining erythrocyte diagnosticum for reaction of indirect hemagglutination (RIHG) in case of cattle coccielosis consists of fractional formalinisation of sheep erythrocytes and their sensibilisation with coccielosis antigen at 70°C for 30 minutes. For sensibilisation used are erythrocytes, loaded with sensitin obtained from vaccine strain C.burnetii M-44, heated on water bath at 70°C for 30 minutes, with further triple washing of erythrocytic diagnosticum with buffer solution with pH -7.2.
EFFECT: invention ensures increase of specificity and activity of diagnosticum in RIHG in case of cattle coccielosis.
2 tbl
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METHOD FOR PREPARING ERYTHROCYTIC DIAGNOSTICUM FOR INDIRECT HEMAGGLUTINATION TEST (IHGT) IN CATTLE ANAPLASMOSIS | 2007 |
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Authors
Dates
2011-01-27—Published
2009-05-26—Filed