PHAGE DISPLAY WITH APPLICATION OF COTRANSLATIONAL TRANSLOCATION OF FUSED POLYPEPTIDES Russian patent published in 2011 - IPC C12N15/10 C12N15/63 

FIELD: medicine.

SUBSTANCE: invention relates to method of display with application of filamentous phage, as well as to phage or phagmid vectors and libraries of such vectors, applied in the method. Method lies in constituting vector in form of filamentous phage or phagmid, containing expression cassette for fused polypeptide, which has terminal signal sequence, selected from group of signal sequences TorT, SfmC, TolB and DsbA. After that, combinatorial library of phage or phagmid vectors is constructed by cloning library of DNA, coding analysed polypeptides in expression cassette of obtained at previous stage vector, corresponding Gram-negative bacteria are transformed by such library and selection cycle is realised by method of phage display.

EFFECT: method makes it possible to display polypeptides, about which it is known that they can be badly displayed on phages, as well as for proteins of cDNA libraries and other combinatorial libraries, especially those, which are formed as a result of very fast packing, stable protein support.

5 cl, 6 dwg, 3 tbl, 1 ex