FIELD: medicine.
SUBSTANCE: invention relates to construction of transposon-induced mutant of melioidosis causative agent Burkholderia pseudomallei, bearing inactivated sequences of genes of drug efflux (multidrug efflux pumps) and chromosomal class A β-lactamases. The strain is obtained as a result of directed genetic construction, including stages of insertion mutagenesis of cells of polyresistant strain B. pseudomallei 57576 SMCP with high level of resistance to cephalosporins and fluoro-quinolones. The strain is deposited in SC "Microbe" under No KM31. In accordance with the invention the strain is a stable mutant, bearing insertions Tn9 in sequences of genes blaD, omp27 and omp71, preserving its properties during storage, cultivation in nutrient media and during passages through organism of laboratory animals, and intended for investigation of role of said R-determinant in development of drug resistance of multiple type in melioidosis causative agent and closely related microorganisms.
EFFECT: strain KM 31 can be used for investigation of functional role of genes of antibiotic efflux and determinants of functional modification, inactivation of antimicrobial drugs in pathogenic species of genus Burkholderia.
3 dwg, 2 tbl, 3 ex
