METHOD FOR DETECTING EXPRESSING GENES OF PATHOGENIC BURCHOLDERIA BY DIFFERENTIAL DISPLAY Russian patent published in 2012 - IPC C12Q1/68 C12N1/20 C12N15/10 

FIELD: medicine.

SUBSTANCE: invention discloses a method for detecting the expressing genetic determinants of the pathogenic microorganisms Burkholderia with using reverse transcription and conjugated amplification (RT-PCR) for the following structure functional analysis. The method involves the stages of bacterial culture growing to an early logarithmic growth phase, RNA recovery and purification by nucleosorption from the sodium merthiolate processed broth culture, removal of DNA impurities by DNA-ase processing, cDNA synthesis in the presence of a ribonuclease and primer inhibitor specific to sequences of procaryote mRNA ribosome determinants, cDNA reamplification with the same primer for collection and use thereof as a matrix in gene-specific PCR, recovery and detection of the nucleotide sequence of amplicons.

EFFECT: method provides assessing the action of cell adaptation variations in response to the varying environmental conditions on the manifestations of the most important biological characteristics of microorganisms - virulence and antimicrobial resistance - with a high degree of sensitivity at various environmental conditions.

3 dwg, 3 ex