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2 608 506

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IPC

C12N1/00(2006-01-01)

C12Q1/68(2006-01-01)

(21) (22)

Application

2016103419, 2016-02-02

(24)

Start date

2016-02-02

(22)

Actual filing date

2016-02-02

(45)

Published

2017-01-18

(72)

Inventor

Viktorov Dmitrij ViktorovichZakharova Irina BorisovnaKuzyutina Yuliya AleksandrovnaLopastejskaya Yana Anatolevna

(73)

Holder

Federalnoe Kazennoe Uchrezhdenie Zdravookhraneniya Nauchno-Issledovatelskij Protivochumnyj Institut" Federalnoj Sluzhby Po Nadzoru V Sfere Zashchity Prav Potrebitelej I Blagopoluchiya Cheloveka

SET OF 5'-PHOSPHORYLATED OLIGONUCLEOTIDE PRIMERS FOR AMPLIFICATION BY POLYMERASE CHAIN REACTION OF COMPLETE CODING SEQUENCE OF MEMBRANE PROTEIN TTSS HRCV OF BURKHOLDERIA PSEUDOMALLEI Russian patent published in 2017 - IPC C12N1/00 C12Q1/68

Abstract RU 2608506 C1

FIELD: biology.

SUBSTANCE: invention relates to molecular biology and biotechnology. Invention represents set of 5'-phosphorylated oligonucleotide primers for amplification by polymerase chain reaction (PCR) of complete coding sequence of membrane protein of secretion system (TTSS) HrcV type III of causative agent of melioidosis.

EFFECT: invention allows to produce high concentration of DNA fragment, carrying complete sequence of gene, coding highly immunogenic membrane protein TTSS HrcV of causative agent of melioidosis, which allows to perform in future its cloning in expressing vector for purpose of accumulation of recombinant antigen.

1 cl, 1 dwg, 2 tbl, 2 ex

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RU 2 608 506 C1

Authors

Viktorov Dmitrij Viktorovich

Zakharova Irina Borisovna

Kuzyutina Yuliya Aleksandrovna

Lopastejskaya Yana Anatolevna

Dates

2017-01-18—Published

2016-02-02—Filed