METHOD OF STUDYING RELIEF-FORMING STRUCTURES OF BIOLOGICAL MEMBRANES Russian patent published in 2011 - IPC G01N33/483 G01N1/30 

FIELD: medicine.

SUBSTANCE: separated films are placed for 6 hours into 30% water solution of alcohol in amount, equal to preparation volume. After that, they are washed in flowing water for 10 minutes in order to remove let down mesothelial cells, fixed in formalin, washed and placed into 96% alcohol for 30 minutes to degrease the surface, after that, sensitised in 10% solution of silver nitrate for 10 minutes. Then silver is impregnated by keeping preparation for 30 minutes in ammonium solution of silver nitrate, washed for 2 minutes in 5 parts of distilled water to remove impregnated silver from basal membrane, remaining impregnated silver is reduced into metal state with 1% formalin during 30 seconds to give preparation blackish colour. Microscopy is carried out on unfolded wet free film preparations by means of microscopes with flat field of incident light, provided with objectives for work without cover glasses, at unilateral oblique incident illumination, carrying out for said purpose edge blocking half of light flow of standard-issue episystem of microscope illumination in mode of dark field with non-transparent shutter.

EFFECT: method improvement.

3 cl, 11 dwg