METHOD TO DETECT SARCOCYST TROPHOZOITES Russian patent published in 2011 - IPC G01N33/00 G01N33/02 G01N33/48 

FIELD: veterinary science.

SUBSTANCE: artificial gastric acid is composed according to prescription: distilled water of temperature 41-42°C - 1000.0 ml, concentrated hydrochloric acid with specific weight of 1.2 - 12.0-15.0 ml, porcine food pepsin 20-30 g. Incubation is carried out in "Gastros" device at the temperature of 41-42°C. Broth is settled, afterwards the residue is centrifuged. Then 8.0 ml of upper fluid level is aspirated from test tube, a drop is taken from lower level, and a smear is made on slide plate. Remaining part is applied with a dropper onto slide plates and dried, then made preparations are stained according to Romanovskiy-Giemsa for 10 minutes and are investigated under microscope with increase of ×400 and ×1250 with immersion.

EFFECT: method is convenient and simple to use, makes it possible to validly detect sarcocyst trophozoites.

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