FIELD: biotechnologies.
SUBSTANCE: invention relates to the biotechnology. Disclosed is a method for preparing crop cultures of anthrax microbe. Method comprises culturing a reference culture of Bacillus anthracis STI-1 strain using a deep-well method at temperature of 32–34 °C for 36–40 hours during aeration and stirring using culture media based on fish course hydrolysates. When 70–75 % freely spores and forespores are found in culture, content of fermenter is heated to 36–37 °C and held without aeration for 3–5 hours to achieve 90 % spores stained by Ziehl-Nielsen, then concentrated and washed native culture.
EFFECT: method provides increased quantity of seed grass.
1 cl, 1 dwg, 2 tbl, 2 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF PREPARING NATIVE SPORE SUSPENSION FOR ANTHRAX VACCINE PREPARING | 2001 |
|
RU2198921C2 |
| METHOD OF SUBMERGED CULTURING ANTHRAX VACCINE STRAIN STI-1 | 1997 |
|
RU2142010C1 |
| ATTENUATED STRAIN Bacillus anthracis FOR DEVELOPING MEANS OF SPECIFIC PREVENTION OF ANTHRAX | 2013 |
|
RU2544951C1 |
| METHOD FOR PRODUCING DIAGNOSTIC ANTHRAX ALLERGEN | 2009 |
|
RU2415941C1 |
| MIXED ANTHRAX VACCINE | 1992 |
|
RU2115433C1 |
| METHOD OF PRODUCTION OF PROTECTIVE ANTIGEN AND PROTEIN OF S-LAYER EA1OF ASPOROGENOUS RECOMBINANT STRAIN IN anthracis 55 ΔTPA-1Spo | 2012 |
|
RU2492241C1 |
| ANTHRAX VACCINE STRAIN STI-PR-4 WITH BROADENED SPECTRUM OF RESISTANCE TO ANTIBIOTICS | 2000 |
|
RU2173342C1 |
| METHOD OF CONCENTRATING SPORE CULTURE IN PRODUCTION OF ANTHRAX VACCINE PREPARATIONS | 1998 |
|
RU2151798C1 |
| DNA-STRUCTURE ENCODING MODIFIED VERSION OF PROTECTIVE ANTIGENE BACILLUS ANTHRACIS | 2015 |
|
RU2622085C2 |
| TEST-INFECTING CULTURE IN ASSAY OF IMMUNOGENICITY OF ANTHRAX VACCINES, EFFECTIVENESS OF AGENTS FOR EMERGENCY PROPHYLAXIS AND TREATMENT OF PATIENTS WITH ANTHRAX | 1997 |
|
RU2141522C1 |
