FIELD: medicine.
SUBSTANCE: lipoaspirate-isolated mesenchymal stromal cells (lMSC) are pre-cultivated in conditions of hypoxia 5% O2. After that, in conditions of hypoxia: 1-3% in case of necessity to obtain a large number of cells during 1-2 passages or with 5% O2 in case when longer cultivation is possible.
EFFECT: invention makes it possible to obtain a large number of MSC during a short period of time.
2 cl, 3 dwg, 3 tbl, 1 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF REPRODUCING HUMAN MESENCHIMAL STROMAL CELL CULTURES RECOVERED FROM LIPOASPIRATE | 2007 |
|
RU2351649C1 |
| METHOD FOR FASTER FORMATION OF CALLUS IN MAMMALS | 2009 |
|
RU2404242C1 |
| METHOD OF ACCELERATED ACQUIRING OF MESENCHYMAL STEM CELLS (MSC) CULTURE OF MAMMALS, EXCEPT HUMAN, WITH LOW HETEROGENEITY AND HIGH VIABILITY | 2007 |
|
RU2331670C1 |
| METHOD FOR STIMULATING FORMATION OF FIBROCARTILAGENOUS REGENERATED CLAVUS IN MAMMALS | 2011 |
|
RU2461621C1 |
| Ex vivo METHOD TO INCREASE QUALITY OF PRECURSOR CELLS FROM AGE DONORS | 2012 |
|
RU2484133C1 |
| METHOD FOR CORD BLOOD MONONUCLEAR CELLS (cbMNC) EXPANSION ex vivo IN PRESENCE OF MULTIPOTENT MESENCHYMAL STROMAL CELLS (MMSCS) | 2013 |
|
RU2525143C1 |
| METHOD FOR EVALUATING AND MONITORING IN VITRO PURITY OF A CELL PREPARATION, USE OF A GROWTH FACTOR, A KIT | 2016 |
|
RU2738969C2 |
| METHOD FOR PRODUCING ASSOCIATES OF HEMATOPOIETIC AND STROMAL PROGENITOR CELLS CAPABLE OF SUPPRESSING ACTIVATION AND PROLIFERATION OF ALLOGENIC LYMPHOCYTES | 2019 |
|
RU2722669C1 |
| COMPOSITION FOR PREVENTIVE OR THERAPEUTIC TREATMENT OF CONDITIONS ASSOCIATED WITH CHANGING AMOUNT OF HAIR | 2018 |
|
RU2716159C2 |
| METHOD FOR OBTAINING OF MSC-ASSOCIATED NON-DIFFERENTIATED HEMOPOIETIC PRECURSOR CELLS WITH CD34+/CD133+ PHENOTYPE | 2016 |
|
RU2628092C1 |
