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2 525 143

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IPC

C12N5/02(2006-01-01)

C12N5/07(2010-01-01)

C12N5/786(2010-01-01)

C12N5/797(2010-01-01)

(21) (22)

Application

2013112801/10, 2013-03-22

(24)

Start date

2013-03-22

(22)

Actual filing date

2013-03-22

(45)

Published

2014-08-10

(72)

Inventor

Buravkova Ljudmila BorisovnaAndreeva Elena Romual'DovnaMaslova Elena ViktorovnaRomanov Jurij Askol'DovichGrigor'Ev Anatolij Ivanovich

(73)

Holder

Federal'Noe Gosudarstvennoe Bjudzhetnoe Uchrezhdenie Nauki Gosudarstvennyj Nauchnyj Tsentr Rossijskoj Federatsii-Institut Mediko-Biologicheskikh Problem Rossijskoj Akademii Nauk

METHOD FOR CORD BLOOD MONONUCLEAR CELLS (cbMNC) EXPANSION ex vivo IN PRESENCE OF MULTIPOTENT MESENCHYMAL STROMAL CELLS (MMSCS) Russian patent published in 2014 - IPC C12N5/02 C12N5/07 C12N5/786 C12N5/797

Abstract RU 2525143 C1

FIELD: medicine.

SUBSTANCE: what is presented is a method for cord blood mononuclear cells (cbMNC) expansion ex vivo in the presence of multipotent mesenchymal stromal cells (MMSCs) involving culturing MMSCs from the stromal vascular fraction of fatty tissue to reach a monolayer at the concentration of O₂ in the medium of 5%, adding a cbMNC suspension to the MMSCs monolayer, culturing for 72 hours at the concentration of O₂ in the medium of 5%, selecting the unattached cbMNCs and replacing the medium, continuing the MMSCs culturing with the attached cbMNCs for 7 days at the concentration of O₂ in the medium of 5%.

EFFECT: invention can be used in preparing the stem cell cultures for needs of regeneration medicine.

3 dwg, 3 tbl, 1 ex

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RU 2 525 143 C1

Authors

Buravkova Ljudmila Borisovna

Andreeva Elena Romual'Dovna

Maslova Elena Viktorovna

Romanov Jurij Askol'Dovich

Grigor'Ev Anatolij Ivanovich

Dates

2014-08-10—Published

2013-03-22—Filed