FIELD: medicine.
SUBSTANCE: recombinant plasmid pESAT6-CBD consisting of an artificial bacterial operon of chimeric protein containing a promotor area of an early promotor of bacteriophage T5, chimeric protein gene and transcription terminator, a bacterial operon of beta-lactamase and a bacterial ColEl-type replication initiation site is described. The invention also involves the Escherichia coli strain - producer of chimeric protein ESAT6-CBD, and also a method of immobilisation, concentration and cellulose purification of produced protein. Besides, the invention refers to the same recombinant protein ESAT6-CBD and an immunogenic composition containing it and used for tuberculous infection immunity induction.
EFFECT: invention allows producing the producer strain providing a high production level of resistant immunogenic proteins which can be one-stage produced, immobilised and purified, producing the effective immunogenic compositions for tuberculosis.
6 cl, 1 dwg, 5 ex
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